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Enzymatic THC Forum |
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Subject :Re: Whattup making Enzymatic THC ..
17-03-2009 08:07:05
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Subject :Re: Whattup making Enzymatic THC ..
16-03-2009 12:34:58
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Forum :
Discussion on how to produce THC enzymatically
Topic :
Whattup making Enzymatic THC
Hi - it'great that you're interested in this subject, however your questions leave me guessing you are **very** far from accomplishing this work in your home. maybe you can put your head together with some of your friends who know more about biochemistry and get going with this - and believe me, I wish you the best of luck - it's just that right now i'm not going to spend 100+ hours breaking down the process and the documents. If you need a particular journal I suggest you go to a library - that's where I spend a lot of time. I'm not trying to be rude, it's just that you've got a long way to go based on what you're telling me. I'll leave this forum here and you and your friends are welcome to use it as you wish. Alternately I'm blogging on here http://www.tangler.com/forum/greenstone-enzymatic-thc-forum about somewhat related subjects... Best of luck man, and be safe |
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Subject :Re: Whattup making Enzymatic THC ..
16-03-2009 01:48:15
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Forum :
Discussion on how to produce THC enzymatically
Topic :
Whattup making Enzymatic THC
Hi, Does it mean that if I have the bacteria E coli M15, that I have somehow the appropriate reagent,3 MalonylCo-A & 1n-Hexanoyl-CoA OSCoA, then I should get after proper brewing the products, OSCoA and OlivetolicAcid? How do I get the reagents? Can one buy E coli M15? Sorry I do not have access to the paper of Raharjo, 2004 Is it possible to have them online somehow? Best regards |
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Subject :Re: Whattup making Enzymatic THC ..
15-03-2009 10:03:04
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Forum :
Discussion on how to produce THC enzymatically
Topic :
Whattup making Enzymatic THC
For those who may pass through here... this is the "official" recipe - should put you near the path [0040] Table 1 Enzyme | Reagents | Product | Time | Temp | Vector | KeyReferences | Accession# | Polyketide STCSL/ CHS | 3 MalonylCo-A & 1n-Hexanoyl-CoA OSCoA | OSCoA OlivetolicAcid | 1-24 hrs | 25-35 C | E. Coli M15 | Raharjo, 2004 | AY082343 | CBDAs | CannabigerolicAcid | CannabichromenicAcid | 24-48hrs | 25-35C | TobaccoRoot Hairs | Morimoto,1999 | | Prenylase | OlivetolicAcid + GPP | CannabigerolicAcid | 1-24hrs | 25-35C | E.Coli M15 | Fellermeier,1998 | | CBCAs | CannabigerolicAcid | CannabidiolicAcid | 24-48hrs | 25-35C | TobaccoRoot Hairs | Morimoto,1999 | | THCAs | CannabigerolicAcid | TetrahydrocannabinolicAcid | 24-48hrs | 25-35C | TobaccoRoot Hairs | Taura,1995 & Sirikantaramas 2004 | AB057805 | |
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Subject :Re: Whattup making Enzymatic THC ..
15-03-2009 09:12:36
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Subject :Re: Whattup making Enzymatic THC ..
15-03-2009 07:57:16
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Subject :Whattup making Enzymatic THC ..
13-03-2009 07:35:21
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Forum :
Discussion on how to produce THC enzymatically
Topic :
Whattup making Enzymatic THC
Recently receiving a lot of email - summed up clearly in this excerpt from one clever geek, >>To be able to produce THC in enzimatic process without growing the plant seems to me a wonderful finding. >>I read your patent few times, but poorlly understood the operation or procedure described in it. >>Did you realize any experiment that lead to THC? >>Is it something that can be done with off the shelves reagent? >>I would like to have somehow a "how to" procedure in order to realize it in my lab at home. >>I do not intend to commercialize anything it is only for personnal use and enjoyment. So yeah - couldn't agree more - let's work on a constructive discussion to elaborate and enlighten upon this work This forum is here to cover as many of these topics in the depth that may be required to take a newbie through the ropes to recreate this patent for personal use - recall that the USPTO allows the recreation of any patent for personal non-commercial use - such is its beauty and bounty. Enjoy. |
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