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Enzymatic THC Forum |
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Subject :Re: Whattup making Enzymatic THC ..
17-03-2009 08:07:05
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Subject :Re: Whattup making Enzymatic THC ..
16-03-2009 12:34:58
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Subject :Re: Whattup making Enzymatic THC ..
16-03-2009 01:48:15
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| vincent |
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| Junior |
   
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Joined: 2009-03-15 14:51:14
Posts: 3
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Hi, Does it mean that if I have the bacteria E coli M15, that I have somehow the appropriate reagent,3 MalonylCo-A & 1n-Hexanoyl-CoA OSCoA, then I should get after proper brewing the products, OSCoA and OlivetolicAcid? How do I get the reagents? Can one buy E coli M15? Sorry I do not have access to the paper of Raharjo, 2004 Is it possible to have them online somehow? Best regards |
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Subject :Re: Whattup making Enzymatic THC ..
15-03-2009 10:03:04
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| admin |
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| Junior |
   
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Joined: 2009-03-11 19:59:01
Posts: 4
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For those who may pass through here... this is the "official" recipe - should put you near the path [0040] Table 1 Enzyme | Reagents | Product | Time | Temp | Vector | KeyReferences | Accession# | Polyketide STCSL/ CHS | 3 MalonylCo-A & 1n-Hexanoyl-CoA OSCoA | OSCoA OlivetolicAcid | 1-24 hrs | 25-35 C | E. Coli M15 | Raharjo, 2004 | AY082343 | CBDAs | CannabigerolicAcid | CannabichromenicAcid | 24-48hrs | 25-35C | TobaccoRoot Hairs | Morimoto,1999 | | Prenylase | OlivetolicAcid + GPP | CannabigerolicAcid | 1-24hrs | 25-35C | E.Coli M15 | Fellermeier,1998 | | CBCAs | CannabigerolicAcid | CannabidiolicAcid | 24-48hrs | 25-35C | TobaccoRoot Hairs | Morimoto,1999 | | THCAs | CannabigerolicAcid | TetrahydrocannabinolicAcid | 24-48hrs | 25-35C | TobaccoRoot Hairs | Taura,1995 & Sirikantaramas 2004 | AB057805 | |
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Subject :Re: Whattup making Enzymatic THC ..
15-03-2009 09:12:36
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Subject :Re: Whattup making Enzymatic THC ..
15-03-2009 07:57:16
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Subject :Whattup making Enzymatic THC ..
13-03-2009 07:35:21
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